Experiment 1025984
Worktypes
Compounds isolated
Details
| Property | Value |
|---|---|
| Pharmacology | |
| Organism | Empty organism |
| Citation | ONO,K: IWATA,Y: NAKANE,H:. (1982). ALLOSTERIC INHIBITION BY APHIDICOLIN OF THE ACTIVITY OF DNA POLYMERASE ALPHA FROM MOUSE MYELOMA.. 11() |
| Amount utilized | |
| Compound isolated | |
| Disease | THE MECHANISM OF INHIBITION BY APHIDICOLIN OF THE ACTIVITY OF PURIFIED DNA POLYMERASE ALPHA WAS STUDIED. IN THE PRESENCE OF APHIDICOLLIN + ACTIVATED DNA,DNA POLYMERASE ALPHA FROM MOUSE MYELOMA DID NOT SHOW MICHAELIS KINETICS WITH RESPECT TO CHANGES IN NUCLEOTIDE SUBSTRATE CONCENTRTION(I.E. DGTP + DTTP). A PLOT OF THE REACTION VELOCITY VS.DGTP OF DTTP CONCENTRATION WAS HYPERBOLIC WITH AN INTERMEDIARY PLATELET. THE DOUBLE RECIPROCAL PLOT WAS CONCAVE DOWNWARDS, AND THE HILL PLOT GAVE HILL COEFFICIENTS OF LESS THAN 1.0 OVER MOST OF THE CURVE. APPARENTLY APHIDICOLIN ACTS AS AN ALLOSTERIC INHIBITOR FOR DNA POLYMERASE ALPHA. DNA POLYMERASE ALPHA FUNCTIONS AS A MULTISITE ENZYME THAT EXHIBITS NEGATIVE COOPERATIVITY AMONG AT LEAST THREE BINDING SITES OF THE NUCLEOTIDE SUBSTR ATES(DGTP + DTTP). |
| Number | 1 |
| Qualitative result | ACTIVE |
| Dose expression | CONC USED |