Experiment 1047591

Details

PropertyValue
Pharmacology
OrganismEmpty organism
CitationDOWNS JR,RW: AURBACH,GD:. (1982). THE EFFECTS OF FORSKOLIN ON ADENYLATE CYCLASE IN S49 WILD TYPE AND CYC-CELLS.. 8(4)
Amount utilized
Compound isolated
DiseaseTHE EFFECT OF THE TITLE COMPOUND ON ADENYLATE CYCLASE IN S49 WILD TYPE AND CYC- CELLS WAS TESTED. THE COMPOUND STIMULATED ADENYLATE CYCLASE ACTIVITY IN CYC- MEMBRANES, PARTICULARLY WITH MN2+ AS A COFACTOR. STIMULATION OF ADENYLATE CYCLASE IN WILD TYPE MEMBRANES WAS GREATER THAN IN CYC- MEMBRANES AND THE ABILITY OF FORSKOLIN TO STIMULATE CYC- MEMBRANES WAS ENHANCED BY LUBROL PX EXTRACTS OF HUMAN ERYTHROCYTE MEMBRANES COMPARED TO ITS POTENT EFFECT ON INTACT WILD TYPE CELLS, FORSKOLIN WAS A POOR STIMULATOR OF C-AMP ACCUMULATION IN CYC-CELLS. CYC- CELLS PROLIFERATED IN MEDIUM CONTAINING FORSKOLIN, WHILE THE GROWTH OF WILD TYPE CELLS IN MEDIUM WAS INHIBITED AND THE WILD TYPE CELLS ULTIMATELY DIED. CLONES SELECTED FROM A SUSPENSION OF WILD TYPE CELLS ON THE BASIS OF FORSKOLIN RESISTANCE SHOWED THE CHARACTERISTICS OF CYC- CELLS. THUS, FORSKOLIN DOES NOT SUBSTANTIALLY ACTIVATE ADENYLATE CYCLASE ACTIVITY IN INTACT CYC- CELLS. APPARENTLY, GUANINE NUCLEOTIDE REGULATORY PROTEIN ENHANCES FORSKOLIN ACTIVATION OF ADENYLATE CYCLASE.
Number1
Qualitative resultACTIVE
AnimalCELL CULTURE
Dose expressionCONC USED