Experiment 1217468
Worktypes
Details
| Property | Value |
|---|---|
| Pharmacology | |
| Administration route | IP |
| Organism | Celastrus paniculatus (CELASTRACEAE) |
| Citation | BIDWAI,PP: WANGOO,D:. (1987). THE INVOLUTORY EFFECTS OF SEED EXTRACT OF CELASTRUS PANNICULATUS ON THE EPIDIDYMIS AND VAS DEFERENS OF ALBINO RATS.. 2(2) |
| Amount utilized | |
| Compound isolated | |
| Disease | THE EXCURRENT DUCTS OF MATURE MALE ALBINO RATS WERE STUDIED AFTER TREATMENT WITH AN OIL EXTRACTED FROM THE TITLE PLANT. RATS WERE INJECTED WITH THE OIL EVERY 48 HRS FOR 30 DAYS. AFTER A POST-TREATMENT REST OF 10, 20 & 30 DAYS RESPECTIVELY, TREATED + CONTROL RATS WERE SACRIFICED AND THE EPIDIDYMIS AND VAS DEFERENS WERE DISSECTED OUT AND EXAMINED HISTOLOGICALLY, HISTOCHEMICALLY AND BIOCHEMICALLY. THE WEIGHT OF THE EPIDIDYMIS SHOWED SIGNIFICANT DECREASE. THERE WAS A HIGHLY SIGNIFICANT DECREASE IN TOTAL LIPIDS, CHOLESTROL AND DNA. RNA AND PHOSPHOLIPIDS WERE SIGNIFICANTLY INCREASED. HISTOPHYSIOLOGICAL REGRESSION WAS OBSERVED RESULTING IN 1) SIZE OF THE DUCTS, 2) EPITHELIAL CELL HEIGHT AND 3) AMOUNT OF SEMINAL FLUID. VAS DEFERENS SHOWED SIMILAR FEATURES OF REGRESSION TO EPIDIDYMIS AND ALSO A DESQUAMATION OF ITS EPITHELIUM. BEFORE THE LAST STAGES OF REGRESSION IN GROUP 3, THE VAS DEFERENS EPITHELIUM IN GROUPS 1 AND 2 SHOWED PROGRESSIVE SHIFT OF THE NUCLEI TOWARD AN ADLUMINAL SURFACE OF THE TALL CELLS. THESE CELLS WERE DISCHARGED LEAVING MOSTLY THE BASAL CELLS. ABSTRACTORS NOTE: THERE DOES NOT SEEM TO BE ANYTHING ABNORMAL IN THE HISTOLOGY PHOTOGRAPHS IN THE ARTICLE. IT IS DIFFICULT TO EXPLAIN "EFFECTS" THAT WERE GREATEST 30 DAYS AFTER TREATMENT WAS STOPPED. |
| Dose amount | 12.0 ML |
| Number | 1 |
| Qualitative result | . |
| Animal | RAT |
| Per unit | ANIMAL |
| Dose expression | DOSE |
| Extract | SEED OIL |
| Gender | MALE |