Experiment 1227065
Compounds isolated
Details
| Property | Value |
|---|---|
| Pharmacology | |
| Administration route | INTRAGASTRIC |
| Organism | Empty organism |
| Citation | WANG,NG: GUAN,MZ: LI,HP: LEI,HP:. (1987). EFFECT OF GOSSYPOL ACETIC ACID (GAA) AND 15-METHYLPGF2-ALPHA-METHYL ESTER (PG05) ON ESTROGEN RECEPTOR OF RAT UTERI.. 22(2) |
| Amount utilized | |
| Compound isolated | |
| Disease | TREATMENT OF PSEUDOPREGNANT RATS WITH GOSSYPOL FOR 5 DAYS CAUSED A DECREASE OF CYTOPLASMIC PROTEIN AND ESTROGEN RECEPTOR LEVEL PER UTERUS, EVEN THOUGH THE ESTROGEN RECEPTOR CONCENTRATION PER MG PROTEIN DID NOT SHOW SIGNIFICANT CHANGES BETWEEN THE CONTROL AND TREATED ANIMALS. SATURATION ANALYSIS SHOWED THAT IMMATURE RATS TREATED WITH GOSSYPOL FOR 8 DAYS REDUCED THE AMOUNT OF ESTROGEN RECEPTOR PER UTERUS. BMAX IN THE CONTROL AND TREATED GROUPS WAS FOUND BY SCATCHARD ANALYSIS TO BE 432.0 AND 358.8 FMOL/UTERUS, RESPECTIVELY. THE IC50 OF GOSSYPOL WAS SHOW TO BE 7.0 X 10 (-5) MOL INDICATING THAT GOSSYPOL AT HIGH CONCENTRATIONS WAS CAPABLE OF INHIBITING THE BINDING OF 3H-ESTRADIOL WITH THE RECEPTOR. |
| Dose amount | 40.0 MG |
| Number | 1 |
| Qualitative result | ACTIVE |
| Animal | RAT(PREGNANT) |
| Per unit | KG |
| Dose expression | DOSE |