Experiment 584758

Details

PropertyValue
Pharmacology
OrganismSerenoa repens (ARECACEAE)
CitationDELOS,S: CARSOL,JL: GHAZAROSSIAN,E: RAYNAUD,JP: MARTIN,PM:. (1995). TESTOSTERONE METABOLISM IN PRIMARY CULTURES OF HUMAN PROSTATE EPITHELIAL CELLS AND FIBROBLASTS.. 55()
Amount utilized
Compound isolated
DiseaseWE COMPARE TESTOSTERONE (T) METABOLISM IN PRIMARY CULTUARES OF EPITHELLAL CELLS AND FIBROBLASTS SEPARATED FROM BENIGN PROSTATE HYPERTROPHY (BPH) AND PROSTATE CANCER TISSUES. IN ALL CULTURES, ANDROSTENEDIONE(^4) FORMED BY OXIDATION OF T BY 17 BETA-HYDROXYSTEROID DEHYDROGENASE (17-BETA-HSD) REPRESENTED 80% OF THE METABOLITES RECOVERED. THE AMOUNTS OF 5-ALPHA-DIHYDROTESTOSTERONE (DHT), FORMED BY REDUCTION OF T BY 5-ALPHA-REDUCTASE (5-ALPHA-R), WERE SMALL: 5 AND 2% (BPH) AND 8 AND 15% (ADENOCARCINOMA) FOR EPITHELIAL CELLS AND FIBROBLASTS. RESPECTIVELY. NOTHERN BLOT ANALYSIS OF TOTAL RNA FROM EPITHELIAL CELLS (BPH OR ADENOCARCINOMA) ATTRIBUTED THE REDUCTIVE ACTIVITY TO THE 5-ALPHA REDUCTASE TYPE 1 ISOZYME AND OXIDATIVE ACTIVIT TO THE 17-BETA-HSD TYPE 2. IN CANCER FIBROBLASTS, ONLY LITTLE 17-BETA-HSD TYPE 2 MRNA WAS DETECTED. THE 5-ALPHA-REDUCTASE INHIBITORS, 4-MA (17-BETA-(N,N-DIETHYL)CARBAMOYL-4-METHYL-4-AZA-5-ALPHA-ANDROSTAN-3-ONE) AND FINASTERIDE, INHIBITED DHT FORMATION WITH A PREFERENTIAL ACTION OF 4-MA ON EPITHELIAL CELLS (BPH OR ADENOCARCINOMA) AND OF FINASTERIDE ON FIBROBLASTS FROM ADENOCARCINOMA. NEITHER INHIBITOR ACTED ON ^4 FORMATION. ON THE OTHER HAND, THE LIPIDO-STEROL EXTRACT OF SERENOA REPENS (LSESR, PERMIXON*) INHIBITED THE FORMATION OF ALL THE T METABOLITES STUDIED [IC,- 40 AND 200ML/ML (BPH) AND 90 AND 70 MG/ML (ADENOCARCINOMA) IN EPITHELIAL CELLS AND FIBROBLASTS. RESPECTIVELY]. THESE RESULTS HAVE IMPORTANT THERAPEUTIC IMPLICATIONS WHEN SELECTING APPROPRIATE TREATMENT OPTIONS FOR BPH.
Dose amountVAR
Number1
Qualitative resultACTIVE
AnimalCELL CULTURE
Dose expressionCONC USED
ExtractHEXANE EXT