Experiment 69613

Worktypes

Details

PropertyValue
Pharmacology
Administration routeORAL
OrganismHypericum perforatum (CLUSIACEAE)
CitationHENNESSAY,M: KELLEHER,D: SPIERS,JP: BARRY,M: KAVANAGH,P: BACK,D: MULCAHY,F: FEELY,J:. (2002). ST JOHN'S WORT INCREASES EXPRESSION OF P-GLYCOPROTEIN: IMPLICATIONS FOR DRUG INTERACTIONS.. 53(1)
Amount utilized
Compound isolated
DiseaseCTION OF THE MULTI DRUG TRANSPORTER P-GLYCOPROTEIN IN PBMCS OF HEALTHY VOLUNTEERS. EFFLUX IN BOTH GROUPS PRODUCING GREATER INTRACELLULAR ACCUMULATION OF RHODAMINE. HOWEVER, THIS EFFECT WAS ATTENUATED FOLLOWING TREATMENT WITH SJW (23.9+/- 15.3% VS 75.4 +/- 16.4% P >0.05. SJW INCREASED EXPRESSION AND ENHANCED THE DRUG EFFLUX FUN ARE EXPRESSED AS MEAN +/- S.D. AND WERE SUBJECTED TO NONPARAMETRIC ANALYSIS.P-GLYCOPROTEIN EXPRESSION INCREASED 4.2 FOLD FROM BASELINE IN SUBJECTS TREATED WITH SJW. THERE WAS NO EFFECT WITH RITONAVIR (5 MICROM) INHIBITED P-GLYCOPROTEIN MEDIATED OBTAINED. VIMENTIN AND IE WERE USED AS CONTROLS. THE PRESENCE OF THE MDR 1 GENE PRODUCT WAS CONFIRMED BY RT-PCR. P-GLYCOPROTEIN MEDIATED DRUG EFFLUX WAS DETERMINED AS A FUNCTION OF RHODAMINE EFFLUX IN THE ABSENCE AND PRESENCE OF RITONAVIR. DATA BLOOD LYMPHOCYTES (PBMCS) WERE ISOLATED BY FICOLL DENSITY GRADIENT CENTRIFUGATION, FIXED AND PERMEABLIZED. CELLS WERE STAINTED WITH A P-GLYCOPROTEIN SPECIFIC ANTIBODY, QUANTIFIED BY FLOW CYTOMETRY AND MEDIAN FLUORESCENCE INTENSITY (MFI) VALUES . HEALTHY VOLUNTEERS WERE RANDOMIZED TO EITHER SJW (0.15%) 600 MG THREE TIMES DAILY FOR 16 DAYS (N=15) OR PLACEBO (N=7). BLOOD SAMPLES WERE OBTAINED FOR P-GLYCOPROTEIN EXPRESSION AND FUNCTION AT BASELINE, 16 AND 32 DAYS POST TREATMENT. PERIPHERAL
Dose amount1.8 GM
Number1
Qualitative resultACTIVE
AnimalHUMAN ADULT
Per unitDAY
Dose expressionDOSE
ExtractHYDRO-ALCOHOLIC EXT
GenderGIVEN TO BOTH SEXES