Experiment 784138
Worktypes
Details
| Property | Value |
|---|---|
| Pharmacology | |
| Organism | Hypericum perforatum (CLUSIACEAE) |
| Citation | GUTMANN,H: BRUGGISSER,R: SCHAFFNER,W: BOGMAN,K: BOTOMINO,A: DREWE,J:. (2002). TRANSPORT OF AMENTOFLAVONE ACROSS THE BLOOD-BRAIN BARRIER IN VITRO.. (68) |
| Amount utilized | |
| Compound isolated | |
| Disease | THE PENETRATION OF [3H]-AMENTOFLAVONE THROUGH BBB WAS STUDIED IN AN IN VITRO MODEL CONSISTING OF PRIMARY CELL CULTURES OF PORCINE BRAIN CAPILLARY ENDOTHELIAL CELLS (BCEC). CONCENTRATION-DEPENDENT UPTAKE (37-200 NM) WAS NEITHER SATURABLE NOR TEMPERATURE-SENSITIVE INDICATING PASSIVE DIFFUSION AS THE MAJOR UPTAKE MECHANISM. THIS FINDING WAS CONFIRMED BY TRANSPORT EXPERIMENTS THROUGH BCEC MONOLAYERS (>2% OF APPLIED DOSE WAS TRANSPORTED AFTER 30 MIN). CO-ADMINISTRATION OF HYPERICUM EXTRACT INCREASED AMENTOFLAVONE TRANSPORT SIGNIFICANTLY INDICATING THAT HYPERICUM CONSTITUENTS ENHANCE AMENTOFLAVONE TRANSPORT POSSIBLY BY MODULATING PARA-CELLULAR PERMEABILITY. EXPERIMENTS WITH THE P-GLYCOPROTEIN (P-GP) OVEREXPRESSING CELL LINE P388-MDR SHOWED THAT AMENTOFLAVONE UPTAKE WAS SIGNIFICANTLY ENHANCED BY ADDITION OF THE P-GP INHIBITORS VERAPAMIL, SUGGESTING A P-GP MEDIATED BACK-TRANSPORT OUT OF THE CELLS. THE BLOOD-BRAIN BARRIER IN VITRO BY PASSIVE DIFFUSION. |
| Dose amount | VAR |
| Number | 1 |
| Qualitative result | ACTIVE |
| Animal | CELL CULTURE |
| Dose expression | DOSE |
| Extract | HYDRO-ALCOHOLIC EXT |